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. Author manuscript; available in PMC: 2020 Mar 7.
Published in final edited form as: Cell Stem Cell. 2019 Jan 31;24(3):419–432.e6. doi: 10.1016/j.stem.2019.01.002

Figure 4. EGF promotes asymmetric division in satellite stem cells.

Figure 4.

(A) Graphic overview of Myf5-Cre/R26R-nTnG satellite cell transplantation into injured NSG mice.

(B-D) Representative images of transplanted satellite cells stained with DAPI (blue), GFP (green), TdTomato (red) and Pax7 (gray).

(E) Graphic overview of cardiotoxin-induced injury and treatment with recombinant EGF in EGFR cKO mice.

(F) Quantification of Pax7-expressing cells on sections from non-injured and regenerating EGFR cKO or Pax7-CreERT2 TA muscles 10 days post injury.

(G) Quantification of Pax7-expressing and (H) Myog-expressing cells on sections from regenerating EGFR cKO or Pax7-CreERT2 TA muscles 10 days post cardiotoxin-induced injury treated with control (saline), or EGF protein.

(B-D and F-H) Error bars represent means ±SEM; p-values: *=<0.05; (B, D) n=3 donor; (C) n=4 donor; (F) n=4 mice; (G-H) n=3 mice.