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. Author manuscript; available in PMC: 2020 Mar 7.
Published in final edited form as: Cell Stem Cell. 2019 Jan 31;24(3):419–432.e6. doi: 10.1016/j.stem.2019.01.002

Figure 6. EGF Stimulation Rescues Polarity Deficits in mdx Satellite Cells.

Figure 6.

(A) Signaling status of p-EGFR (green) in Pax7-expressing (red) and DAPI positive (blue) cells on mdx EDL myofibers at 1h culture in vehicle or EGF containing media.

(B) Quantification of p-EGFR staining in mdx satellite cells on EDL myofibers fixed at 1h culture in vehicle or EGF containing media.

(C) Quantification of abnormal, planar, and apicobasal orientated mitotic spindles and (D) Pard3 localization in satellite cells on mdx myofibers at 36h of culture in vehicle or EGF containing media.

(E) Quantification of asymmetric divisions relative to total satellite stem cell divisions in WT and mdx myofibers at 42h of culture in vehicle or EGF containing media.

(F) Number of asymmetric divisions per myofiber in WT and mdx myofibers at 42h of culture in vehicle or EGF containing media.

(G) Quantification of Myog-expressing cells per mdx myofiber and (H) total myogenic cells (Pax7- or Myog-expressing cells) per mdx myofiber at 72h of culture in vehicle or EGF containing media.

(I) Graphic overview of cardiotoxin-induced injury and treatment with recombinant EGF in mdx mice.

(J) Quantification of Pax7-expressing and (K) Myog-expressing cells on sections from mdx TA muscles 10 days post injury treated with saline (vehicle), or recombinant EGF.

(B–E and H–J) Error bars represent means ±SEM; p-values: *=<0.05; **=<0.01; ***=<0.005. (B) n=3 mice; (C, E-F) n=3 WT mice and 7 mdx mice; (D) n=3 mice, (G–H) n=3 WT and 5 mdx mice; (J–K) n=4 mice for each group.