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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Dev Biol. 2018 Sep 8;448(2):279–290. doi: 10.1016/j.ydbio.2018.09.005

Figure 6.

Figure 6.

Apoptosis during distal regeneration and wound repair. A, B. TUNEL labeled cells in the epidermal epithelium (ep) and to a lesser extend in the mesenchyme (me) at 1 (A) and 0.5 (B) days after amputation of the oral (A) and atrial (B) siphons. en: endodermal epithelium. Sections are cut perpendicular to the distal-proximal axis of each siphon. C. TUNEL labeled cells the epidermal epithelium (ep) of a wounded oral siphon 1 day after the operation. Yellow pigment cells (Y) congregate in the mesenchyme at the wound site. lm: longitudinal muscle bands. cm: circular muscle bands. Section is cut parallel to the distal-proximal axis of the wounded siphon. Scale bar: 10 μm in A-C. D-E. TUNEL labeled cells at 1 day after neural complex removal. D shows a low magnification image of a section parallel to the wound (W) showing the tissue gap (bracket), and a boxed area at one margin of the gap, which is magnified approximately 3.5X in E to show apoptotic cells in the epidermal epithelium (ep) at the margin of the wound. Y: Yellow pigment cells (Y) aggregate near the wounds. Scale bar: 10 μm in D.