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. 2019 Mar 8;9:3994. doi: 10.1038/s41598-019-40670-y

Figure 5.

Figure 5

Inhibition of C-Jun induces ciliogenesis by decreasing SPRR3 expression in PM2.5-treated RPE cells. (a,b) RPE cells were incubated PM2.5 (25 and 50 µg/mL) for 24 h or anisomycin (0.1 and 1 µg/mL) for 1 h and expression of indicated proteins was detected using western blot analysis. (c,d) RPE cells were treated with PM2.5 (50 µg/mL) in the presence or absence of SP600125 (10 µM) for 24 h. (c) Cells were stained with acetylated tubulin antibody (red) and ARL13B antibody (green) Hoechst dye (blue, yellow: merged image, scale bar = 5 μm). (d) Then, both ciliated cells and cilium length were determined using fluorescence microscopy. (e) RPE cells were exposed to PM2.5 (50 µg/mL) in the presence or absence of SP600125 (10 µM) for 24 h. Then, cells were analyzed using western blotting with indicated antibodies. Data are means ± standard error (SE) of at least three independent experiments (*p < 0.05).