Figure 6.

PM2.5-mediated down-regulation of ciliogenesis and differentiation was restored by inhibition of c-Jun in keratinocytes. (a–c) NHEKs were treated with PM2.5 (25 µg/mL) with or without SP600125 (10 µM) for 24 h (a). Then, cells were stained with acetylated tubulin antibody (red), ARL13B (green), and DAPI (blue, scale bar = 10 μm). Then, both ciliated cells and cilium length were counted under a fluorescence microscopy (b), and harvested to analyze the level of Keratin1 and −10 by using RT-PCR (c). Data are means ± standard error (SE) of at least three independent experiments (*p < 0.05 and **p < 0.01).