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. 2019 Mar 8;9:3945. doi: 10.1038/s41598-019-40388-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro delivery of doxorubicin by MrNVLP into HepG2 cells. HepG2 cells were incubated with free doxorubicin (Dox), virus-like particle of Macrobrachium rosenbergii nodavirus loaded with Dox (MrNVLP-Dox), folic acid (FA)-conjugated-and-Dox-loaded MrNVLP (FA-MrNVLP-Dox), and FA-MrNVLP-Dox added with free FA (FA-MrNVLP-Dox + FA), at equivalent Dox concentration. Untreated cells, cells incubated with MrNVLP, and cells incubated with FA-MrNVLP served as controls.