Fig. 3.

Downregulation of Dr-Trk activity with FR light and kinase inhibitors. a Luciferase assay for Elk-1-dependent transcription in PC6-3 cells co-transfected with pCMVd2-Dr-TrkB, pFR-Luc, and pFA-Elk-1 plasmid mixture (1:100:5) and grown for 30 h under 660 nm or 780 nm light (both 0.5 mW cm⁻²). The indicated kinase inhibitors (100 nM) or dimethyl sulfoxide (DMSO) solvent (0.1%) were added to the culture medium. b Luciferase assay for Elk-1-dependent transcription in PC6-3 cells co-transfected with pCMVd2-Dr-TrkA, pFR-Luc, and pFA-Elk-1 plasmid mixture and grown under 660 nm or 780 nm light. c Luciferase assay for CREB-dependent transcription in PC6-3 cells co-transfected with pCMVd2-Dr-TrkB, pFR-Luc, and pFA-CREB plasmid and grown under 660 nm or 780 nm light in the presence of kinase inhibitors. d Luciferase assay for CREB-dependent transcription in PC6-3 cells co-transfected with pCMVd2-Dr-TrkA, pFR-Luc, and pFA-CREB plasmid mixture and grown under 660 nm or 780 nm light in the presence of kinase inhibitors. Error bars represent s.d., n = 3 experiments