Fig. 6.

Light-dependent neurite outgrowth in PC6-3 cells. a Epifluorescence images of PC6-3 cells transfected with the pEGFP-IRES2-Dr-TrkA plasmid kept for 72 h under either 780 nm (upper panels) or 660 nm (lower panels) light. Before imaging, the cells were fixed and stained with a Dil reagent. Fluorescence images were taken in EGFP (left) and Dil (right) channels. b Quantification of cells bearing neurites from the total number of 50 cells transfected with the pEGFP-IRES2-Dr-TrkA plasmid. c Epifluorescence images of PC6-3 cells transfected with pEGFP-IRES2-Dr-TrkB plasmid kept for 72 h under either 780 nm (upper panels) or 660 nm (lower panels) light. Before imaging, cells were fixed and stained with a Dil reagent. Fluorescence images were taken in EGFP (left) and Dil (right) channels. d Quantification of the cells bearing neurites from the total number of 50 cells transfected with the pEGFP-IRES2-Dr-TrkB plasmid. Error bars represent s.d., n = 3 experiments. Scale bar, 100 µm