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. 2019 Mar 8;9:3928. doi: 10.1038/s41598-019-40222-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) On-demand release of Cas9/gRNA form MENPs surface and plasmid efficacy assessment. An optimized ac-magnetic field (60 Oe for 30 minutes) was applied through an electromagnetic coil to generate enough stimulation to break the bond between Cas9/gRNA and MENP. (B) Bio-functional activity of released Cas9/gRNA will eradicate latently infected HIV reservoirs at cellular level, and (C) an illustration of NF up taken by MG cells via nano-electro-poration. (*p ≤ 0.05; ***p ≤ 0.001; NS-Not Significant).