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. 2019 Mar 8;9:3932. doi: 10.1038/s41598-019-39910-y

Table 2.

Summary of the effect of the tested DES, for the two studied cell lines, after 72 h of exposure. The effect trend concerns the effect of the chemicals in cell viability.

HBA: HBD 1:1 Cell line Effect trend IC50 ± SD (μg.mL−1) LOEC (μg.mL−1)
DES [Chol]Cl: Butanoic acid HaCaT
MNT-1		 Increase
Toxic (slightly)		 —
—		 50
400
[Chol]Cl: Hexanoic acid HaCaT
MNT-1		 Increase
No effect		 —
—		 100
—
[Chol]Cl: Ethylene glycol HaCaT
MNT-1		 Increase
Increase		 —
—		 50
50
[Chol]Cl: 1-Propanol HaCaT
MNT-1		 Increase
Increase		 —
—		 50
50
[Chol]Cl: Urea HaCaT
MNT-1		 Increase
No effect		 —
—		 50
—
[N1111]Cl: Butanoic acid HaCaT
MNT-1		 Increase
Toxic (slightly)		 —
—		 50
500
[N1111Cl: Hexanoic acid HaCaT
MNT-1		 Increase
Increase		 —
—		 50
50
[N1111]Cl: Ethylene glycol HaCaT
MNT-1		 Increase
Increase		 —
—		 50
50
[N1111]Cl: 1-Propanol HaCaT
MNT-1		 Increase
Increase		 —
—		 50
50
[N1111]Cl: Urea HaCaT
MNT-1		 Increase
Increase (slightly)		 —
—		 50
50
[N4444]Cl: Butanoic acid HaCaT
MNT-1		 Toxic effect
Toxic effect		 108.7 ± 4.2
472.6 ± 21.3		 200
50
[N4444]Cl: Hexanoic acid HaCaT
MNT-1		 Toxic effect
Toxic effect		 112.0 ± 4.3
476.1 ± 23.2		 200
100
[N4444]Cl: Ethylene glycol HaCaT
MNT-1		 Toxic effect
Toxic effect		 34.1 ± 5.0
300.9 ± 12.7		 200
200
[N4444]Cl: 1-Propanol HaCaT
MNT-1		 Toxic effect
Toxic effect		 77.9 ± 6.3
250.5 ± 7.6		 200
50
[N4444]Cl: Urea HaCaT
MNT-1		 Toxic effect
Toxic effect		 81.8 ± 3.1
496.7 ± 58.4		 50
200

Inhibitory concentration values (IC50) and Lowest Observed Effect Concentration (LOEC) (Dunnett test following one-way ANOVA; p < 0.05; see Supplementary Tables S1 and S2 for the ANOVA summary) is also presented.