Fig. 4. HLA-DR-dependent cell death in ECs is not mediated via apoptosis or necroptosis.

a, b After pretreatment with IFN-γ for 4 days, HUVECs were incubated with L243 (□) or 50 nM Stauro (o) for 4 h (N = 3). a Cells were subjected to the TUNEL assay as described in Materials and methods. b Activities of caspases were assessed with the Caspase-Glo® 3/7 assay system in a luminometer. c After treatment with zVAD for 30 min in order to block apoptosis, HUVECs were treated with L243 or stauro (50 nM) for up to 4 h (N = 3). d After pretreatment with Nec-1 for 30 min to block necroptosis, HUVECs were incubated with L243 or CHX plus TNF for 4 h (N = 3). Data are presented as mean ± SEM. *P < 0.05. RLU relative light units, Stauro staurosporine