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. 2019 Mar 8;9:4013. doi: 10.1038/s41598-019-40607-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proportion of the winged morph in the Regiella-free (A,C,E) and Regiella-infected (B,D,F) lines treated with antibiotics and exposed to 25 °C (A,B), 28 °C (C,D) and 31 °C (E,F) with different population densities. Regiella-infected lines: R1, R2 and R3. Regiella-free lines: N1, N2 and N3. Symbiont-cured lines treated with antibiotics: R1-C, R2-C, R3-C, N1-C, N2-C and N3-C. ** and * above the bars indicate significant differences between the antibiotic treatment and control at P = 0.01 and 0.05 levels, respectively. ns indicates no significant differences at the P = 0.05 level.