Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 8;9:3979. doi: 10.1038/s41598-019-40482-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TNMD expression during adipogenic differentiation. (a) Gene expression of TNMD at various time points during adipogenic differentiation in human adipose-derived stem cells (ADSCs); mRNA levels were normalized to those of hypoxanthine-guanine phosphoribosyltransferase-1 (HPRT1) and presented as fold-change, as calculated using the Pfaffl method. (b) TNMD protein levels from cell lysates were analyzed by Western blotting using a specific antibody against TNMD (N-14), normalized to the internal control (α-tubulin), and expressed as fold-change; the lower section presents a representative crop blot. (c) Immunofluorescent staining of ADSCs (d0) and differentiated adipocytes at day 14 N-14 terminal domains of TNMD (green) and 4.6-diamidino-2-phenylindole (DAPI; blue; scale bar, 200 μm). All values are expressed as the means ± SEM of three independent experiments. Significant differences were identified using the nonparametric Mann-Whitney U test; *P-value < 0.05.