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. 2019 Mar 8;9:3979. doi: 10.1038/s41598-019-40482-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TNMD triggers inflammation in human differentiated adipocytes. (a) mRNA expression and protein levels of interleukin 1-β (IL-1B); (b) mRNA expression and protein levels of tumor necrosis factor-α (TNF-α), and mRNA levels were normalized to those of hypoxanthine-guanine phosphoribosyltransferase-1 (HPRT1); TNF-α and IL1B protein levels were analyzed by XMap technology (Luminex) as indicated in the methods section. (c) Phospho-NFκB p65 protein levels were analyzed by Western blot using a specific antibody against phospho-NFκB p65, normalized to the internal control (α-tubulin), and expressed as the fold-change. The lower section shows a representative crop blot. The data from three independent experiments are presented as the means ± SEM. Significant differences were identified using the Mann-Whitney U test; *P-value < 0.05.