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. 2019 Mar 8;9:3929. doi: 10.1038/s41598-019-40501-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Principal component analysis plot of biological coefficient of variation (BCV) showing the transcriptome profiles from each of 4 paired (connected by line) E12.5 samples separated clearly by the biological effect of interest i.e. cell types (Dimension 1, X-axis). The effect size of technical batch effect on replicates (Dimension 2, Y-axis) was small, about half of the biological effect. (B) MA-plot of transcriptomic profile in adrenal chromaffin precursor cells versus sympathetic neuroblasts. RNA sequencing analysis of adrenal chromaffin cells and sympathetic neuroblasts revealed 4,786 differential expressed genes with fold change >2 and false discovery rate (FDR) < 0.05 out of 17169 annotated genes, that 2,938 genes expressed higher in adrenal chromaffin cells (red) and 1,848 genes were expressed higher in sympathetic neuroblasts (green). (C,D) The mRNA expression levels of the ten representative cell type-specific marker genes are shown. Markers for chromaffin cells were all expressed at higher level in the adrenal chromaffin cell transcriptomes (C) while markers for neurons were all expressed at higher levels in the sympathetic neuroblast transcriptomes (D).