Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 8;10:1125. doi: 10.1038/s41467-019-08887-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Tobacco smoke and BaP induce PD-L1 in mice, whereas inhibition of PD-L1 suppresses BaP-induced lung cancer. a The A/J mice were exposed to tobacco smoke or treated with BaP for 20 days and sacrificed, the expression PD-L1 in lung tissues was detected by real-time PCR. b The lung tissues were isolated and analyzed by immunohistochemistry using an anti-PD-L1 antibody. Scale bar = 2000 μm. c, d Flow cytometry analysis of PD-L1⁺, CD45^-PD-L1⁺ cells (c) and PD-1⁺ (d) cells in lung tissues of mice exposed to tobacco smoke. PD-1 + cells in lung tissues of mice treated with BaP were also shown (d). PD-1⁺ cells in this experiment were analyzed from the CD45⁺CD3⁺ leukocytes isolated from the same tumor. e The expression of PD-L1 in lung tissues of mice exposed to tobacco smoke was assayed by Western blot. f Lung tissues of BaP-treated mice were isolated and analyzed by IHC assays using an anti-PD-L1 antibody. Scale bar = 2000 μm. g IHC (left and middle panels) and immunofluorescence (right) assays of lung tissues of BaP-treated mice using continuous sections of 5 μm. IHC assay was conducted using anti-TTF1 and anti-PD-L1 antibodies, and immunofluorescence assay was performed using anti-PD-L1 (green) and anti-TTF1 (white) antibodies, and 4,6-diamidino-2-phenylindole (DAPI) to stain the nucleus (blue). IHC assays, scale bar = 2000 μm; immunofluorescence assays, scale bar = 20 μm. h Flow cytometry analysis of PD-L1⁺ and CD45^-PD-L1⁺ cells in lung tissues of mice treated with BaP. i The expression of PD-L1 in lung tissues of BaP-treated mice was assayed by Western blot. j Micro-CT scanning of lungs of BaP-treated mice with or without anti-PD-L1 antibody. k Comparison of tumors in mice of the two groups. l Hematoxylin-eosin (HE) staining of lung sections from BaP-treated mice with or without PD-L1 blockade. Lesions/mouse are shown in (k). Scale bar = 500 μm. m IHC assays of lung tumor tissues of BaP-treated mice using an anti-PD-L1 antibody. Scale bar = 500 μm. n Flow cytometry analysis of CD45⁺CD3⁺CD8⁺ (CD8⁺) cells in tumor tissues of mice treated with BaP and anti-PD-L1 antibody. Student’s t test, *P < 0.05; **P < 0.01. Error bars, sd