Fig. 6.

AhR inhibitor exhibits anti-lung cancer activity in vivo. a C57BL/6 mice were intravenously injected with LLC (5 × 10⁵) cells, and 3 days later the mice were randomized to receive vehicle or ANF treatment. Micro-CT scanning images and HE staining of lung sections are shown. Scale bar = 500 μm. b IHC assays of ANF-treated mice’ lung tumor tissues using indicated antibodies. Scale bar = 500 μm. c Flow cytometry analysis of CD8⁺, CD4⁺, CD45⁺CD3⁺, and CD45⁺B220⁺ cells in the lung tissues. d The expression of PD-L1 in lung tissues was detected by real-time PCR. e The expression of TNFα and IFNγ in the lung tissues was detected by real-time PCR. f Life span of the mice. g, h The C57BL/6 mice were intravenously injected with LLC (5 × 10⁵) cells, and 3 days later randomized to receive vehicle or CH223191 treatment. Micro-CT scanning images (g) and life span of the mice (h) are shown. i, j The NOD/SCID mice were intravenously injected with LLC (5 × 10⁵) cells, and 3 days later randomized to receive vehicle or ANF treatment. Micro-CT scanning images (i) and life span of the mice (j) are shown. k C57BL/6 mice were injected intravenously with LLC (5 × 10⁵) cells and treated with ANF and/or anti-PD-L1 antibody. Micro-CT scanning images, HE and IHC staining of lung sections of the mice are shown. Scale bar = 500 μm. l Flow cytometry analysis of CD8⁺, CD4⁺, and CD3⁺ cells in the lung tissues. m, n The expression of IFNγ (m) and TNFα (n) in the lung tissues was detected by real-time PCR. o Life span of the mice. P values in c-e, l-n, Student’s t test; P values in f, h, j, o, Log-rank test. Error bars, sd