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. 2019 Mar 8;9:3975. doi: 10.1038/s41598-019-40331-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ca²⁺ influx via SOCE activates E-syt1 to reshape punctate ER-PM MCSs into ring-shaped structures under TIRF-SIM. (a–c) Representative TIRF-SIM images of cortical ER in HEK293 cells co-transfected with SNAPf-E-syt1 and STIM1-EGFP (a), EGFP-E-syt1 and mCherry-Sec 61β (b), or SNAPf-E-syt1 and EGFP-KDEL (c). The left panels show images of cells under resting conditions at a lower magnification; boxed areas are magnified and shown on the right. From left to right are cells under resting conditions, store depletion and store replenishment. All these figures are representative of four independent experiments. Scale bars, left 2 μm; right 0.5 μm. Related videos were also shown as Supplementary Videos S2–S4.