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. 2019 Mar 8;9:3914. doi: 10.1038/s41598-019-40328-9

Figure 3.

Figure 3

Effects of EGCG treatment on GAD67 neurons during development of Dp16(1)Yey mice. (A) Representative fluorescent micrographs of immunohistochemically stained GAD67+ neurons in stratum radiatum of wildtype (WT) and Dp16(1)Yey trisomic (TS) adult mice after placebo (Pl), T1, or T2 treatment. White arrows indicate labelled neurons. (B) Quantification of GAD67+ neuron fraction of NeuN+ neurons in sections of stratum radiatum of WT and Dp16(1)Yey adult mice (n = 5). (C) Representative image of slot blots used for protein level quantification: top, GAD67 antibody, bottom, Ponceau staining. (D) Relative GAD67 levels in hippocampus of control or treated WT and TS mice. Two ways ANOVA were performed followed by an Holm–Sidak multiple comparison procedure with *p < 0.05, **p < 0.01.