Table 1.
Primer name | Primer sequence (5′-3′) | Annealing Tm (°C) |
---|---|---|
ObTAT forward | RTHCCTGGHTGGMGBYTWGGTTGG | 55 |
ObTAT reverse | CCTGGAAGRAKKATAACRGATTCCTCY | 55 |
ObHPPR forward | GGATTAGGGTTACCAACACGCC | 45.7 |
ObHPPR reverse | AACAAGGTCAGCCATGGCTTTAC | 45.7 |