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. 2019 Apr;95(4):433–441. doi: 10.1124/mol.118.114561

Fig. 1.

Fig. 1.

FO activates mouse and human TRPA1. (A) Representative calcium imaging traces of neuronal phenotypes observed among 1328 neurons analyzed from DRG cultures of three adult wild-type C57BL/6 mice. The y-axis is the 340/380-nm ratio, a measure of intracellular calcium, and x-axis is the duration of the experiment in minutes. Arrows on x-axis indicate 15-second treatments with each stimulus. AITC, 100 μM allyl isothiocyanate; Cap, 300 nM capsaicin; FO, 1:10,000-fold diluted fennel oil; [K+]o, 30 mM extracellular K+; Me, 400 μM menthol. (B) Response frequency, measured as percentage of cells activated, of FO (1:10,000-fold diluted) responses in presence and absence of 30 μM HC-030031 (HC), a selective TRPA1 antagonist. (C) Representative calcium imaging traces for FO dilutions (x-axis) in normal HEK293 cells (top) and HEK293 cells stably overexpressing human TRPA1 (bottom). (D) Response frequency of FO dilutions in normal (n = 676) and human TRPA1-expressing HEK293 cells (n = 1695).