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. 2019 Apr;95(4):433–441. doi: 10.1124/mol.118.114561

Fig. 2.

Fig. 2.

Anethole elicits calcium response in TRPA1-expressing mouse DRG neurons. (A) Representative calcium imaging traces of neuronal phenotypes observed among 1375 neurons analyzed from DRG cultures of three adult wild-type C57BL/6 mice. The y-axis is the 340/380-nm ratio, a measure of intracellular calcium, and x-axis is the duration of the experiment in minutes. Arrows on x-axis indicate 15-second treatment with given stimuli. AITC, 100 μM allyl isothiocyanate; anethole, 130 μM trans-anethole; Cap, 300 nM capsaicin; FO, 1:10,000-fold diluted fennel oil; [K+]o, 30 mM extracellular K+; Me, 400 μM menthol. (B) Response frequency (n = 964) of 130 μM anethole in presence and absence of 30 μM HC-030031 (HC), a selective TRPA1 antagonist.