Fig. 5.
Anethole induces transmembrane currents in hTRPA1-HEK293 cells. (A) A representative time course of current measured at the indicated holding potentials (Vh) from HEK293 cells stably overexpressing human TRPA1. Anethole was applied at a concentration of 66 μM. (B) I-V relationship of transmembrane current measured before (control) and during administration of anethole (maximum current, Imax). (C) Dose-response relationship. Circles represent mean values of Imax recorded at Vh = 100 mV. Error bars indicate S.E.M. The solid line is the best fit with logistic function. EC50 = 80.8 μM. Shown in parentheses are the number of cells.