Fig. 8.

Repetitive stimulation of TRPA1 by anethole reveals a desensitization of the TRPA1-mediated whole-cell current to the agonist. (A) A representative trace of the whole-cell current recorded from an hTRPA1-HEK293 cell with the plasma membrane potential clamped at −70 mV. Application of anethole (100 µM) is indicated by bars above the trace. (B) Statistical analysis of results illustrated in (A). Magnitude of the second and third responses was normalized to that of the first response. Shown are the mean ± S.E.M. values. *P < 0.05; N.S., not significant (P > 0.05); paired-sample t test, n = 4 cells. Anethole was applied for 1.5 minutes.