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. 2019 Mar 8;61:13. doi: 10.1186/s13028-019-0448-2

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Photomicrographs of extruded disc material from the L2–L3 intervertebral disc in dog no. 25. In this dog, calcification was not detected on preoperative radiographs, neither in the L2–L3 disc space nor in the vertebral canal (see Fig. 4a). a Multiple crystalline fragments of various sizes (arrows) in extruded nucleus pulposus tissue. HE staining (Obj. ×40). b Calcification in the extruded nucleus pulposus, verified by brownish black deposits (arrows). Von Kossa staining (Obj. ×40)