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. 2019 Mar 9;15:22. doi: 10.1186/s13007-019-0406-z

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Colocalization of MPK6 and pERK in microtubule bound spots analyzed by SIM. a–e Microtubule (a), MPK6 (b), pERK (c), MPK6-pERK overlay (d) and microtubule-MPK6-pERK overlay (e) in root epidermal cells of Col-0. f Close-up view of the boxed area of e, showing pERK and MPK6-labeled spots associated with the walls (full arrowheads) or the tips (outlined arrowheads) of cortical microtubules. g–i MPK6 (g), pERK (h) and overlay (i) in spots of the outlined area of b–d. j Fluorescence intensity of the profile drawn in g–i, showing absolute coincidence between the signals corresponding to MPK6 and pERK. Bars in a–e = 5 μm; c = 2 μm; g-i = 1 μm