Fig. 9.

TFE3 fusions promote the expression of 4EBP3 after mTORC1 inhibition. (a to d) 786-O, HK-2, UOK109 and UOK120 cells were treated with mTOR inhibitor (PP242) or DMSO (Control) for 24 h. The expression of 4EBPs were determined by q-PCR, and GAPDH was used as an internal control. (e, f) The expression of 4EBPs were detected by q-PCR after 24 h treatment with mTOR inhibitor (Torin1) or left untreated (Control) in UOK109 and UOK120 cells. (g) The fold-change of 4EBP3 in 786-O, UOK109 and UOK120 cells compared with HK-2. Data were presented as the mean ± SEM. *P < 0.05, **P < 0.01. The unpaired two-tailed Student’s t-test was used to calculate P value