Table 1.
Structural deviation in the backbone torsion angles of the wild type and mutated human FZD4 proteins
| Comparison of wild-type and mutated structures | Major change in backbone torsion angles (residue number) | Major shift in regions | Critical region |
|---|---|---|---|
| P33S | 44,45,110,132-135,137,141-143,147–150 | CRD domain | |
| 285,417–432 | Seven TMs domain. | ||
| 496–537 | K-T-XXX-W, PDZ binding motif | ||
| G36D | 41–46,70,71,133-138,141–150 | CRD domain | |
| 416–431,468 | Seven TMs domain. | ||
| 495–537 | K-T-XXX-W, PDZ binding motif | ||
| E40Q | 135–144,156–158 | CRD domain | |
| 182–186,199, 202 | UCR | ||
| 243–244,248,284-286,337-338,417-420,466–469 | Seven TMs domain | ||
| 505–537 | PDZ binding motif | ||
| Y58C | 134–135,141-144,147–150 | CRD domain | |
| 243–244,248,284-285,334-337,416-431,467-469,495–497 | Seven TMs domain | ||
| 508–537 | PDZ binding motif | ||
| H69Y | 147–149 | CRD domain | |
| 285,287,334-339,415–432 | Seven TMs domain | ||
| 504–537 | K-T-XXX-W, PDZ binding motif | ||
| M105 T | 274–278,284-286,467,468 | Seven TMs domain | |
| 506–537 | PDZ binding motif | 495–537 | |
| M105 V | 107–114,132–135 | CRD domain | |
| 284–286,332-338,416-430,467–469. | Seven TMs domain | ||
| 505–537 | PDZ binding motif | ||
| I114T | 15–44,49,62-81,110,118,134–156 | Signal peptide and CRD domain | |
| 213–214,274–275 | Seven TMs domain | ||
| 495–537 | K-T-XXX-W, PDZ binding motif | ||
| C117R | 49,70-71,73–74 | CRD domain | |
| 274–275 | Seven TMs domain | ||
| 496–537 | K-T-XXX-W, PDZ binding motif | ||
| R127H | 15–44,49,63-81,110-111,118,133–156 | Signal peptide and CRD domain | |
| 173–179 | UCR | ||
| 213–214,274–275 | Seven TMs domain | ||
| 496–537 | K-T-XXX-W, PDZ binding motif | ||
| M157 V | 133–135,142–151 | CRD domain | |
| 284,285,337,338,417-423,428-431,467–470 | Seven TMs domain | ||
| 496–537 | K-T-XXX-W, PDZ binding motif | ||
| C181R | 419–422 | Seven TMs domain | |
| 503–537 | PDZ binding motif | ||
| C181Y | 420–423 | Seven TMs domain | |
| 508–537 | PDZ binding motif | ||
| C204R | 69–72,134,135,144–150 | CRD domain | |
| 337,338,418-420,430,431 | Seven TMs domain | ||
| 495–537 | K-T-XXX-W, PDZ binding motif | ||
| C204Y | 66–67,70-71,102,141–150 | CRD domain | |
| 243–244,419–420 | Seven TMs domain | ||
| 504–537 | K-T-XXX-W, PDZ binding motif | ||
| Y211H | 496–537 | K-T-XXX-W, PDZ binding motif | |
| M223K | 504–537 | K-T-XXX-W, PDZ binding motif | |
| T237R | 214,274–275 | Seven TMs domain | |
| 503–537 | K-T-XXX-W, PDZ binding motif | ||
| R253C | 504–537 | K-T-XXX-W, PDZ binding motif | |
| W335C | 274–275 | Seven TMs domain | |
| 496–537 | K-T-XXX-W, PDZ binding motif | ||
| A370G | 46–52,68-80,141 | CRD domain | |
| 504–537 | K-T-XXX-W, PDZ binding motif | ||
| R417Q | 42–46,147-150,134-137,147–150 | CRD domain | |
| 418–432 | Seven TMs domain | ||
| 507–537 | PDZ binding motif | ||
| G488D | 41–46,109-114,133–151 | CRD domain | |
| 416–432 | Seven TMs domain | ||
| 495–537 | K-T-XXX-W, PDZ binding motif | ||
| G488 V | 38,70 | CRD domain | |
| 505–537 | PDZ binding motif | ||
| S497F | 70–71,73–74 | CRD domain | |
| 503–537 | K-T-XXX-W, PDZ binding motif | ||
| K499E | 147–148 | CRD domain | |
| 284–286, 336–338,418–431, 468–470 | Seven TMs domain | ||
| 505–537 | K-T-XXX-W, PDZ binding motif |
This table shows the impact of FEVR associated missense mutations in the FZD4 protein on its backbone torsion angles by comparing them with its normal structure. The critical region encompasses the K-T-XXX-W and T/S-X-VPDZ binding motifs. In the first column, amino acid residue on the left indicates the wild-type residue; the number shows the amino acid position of the residue in the protein sequence, while the residue on the right shows the mutated residue. The second column specifies the positions at which major structural deviations were observed
The third column depicts the deviated region/residues shared among all mutant proteins analyzed (critical region)
CRD cysteine rich domain, TM transmembrane, UCR uncharacterized region