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. 2019 Mar 8;10:80. doi: 10.1186/s13287-019-1182-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization of USCs and USCs-Exo. A Morphology and growth of USCs. B USCs were characterized by FCM using the surface markers CD29, CD90, CD73, CD44, CD34, CD45, and HLA-DR. C USC osteogenic (C-a) and adipogenic (C-b) differentiation. D Morphology of USCs-Exo under a transmission electron microscope (arrowheads). E TRPS measurement showed that the size range of USCs-Exo concentrated at 70–150 nm, and the measured mean concentration (particles/mL) of USCs-Exo was 1.57 × 10¹¹). F Western blot analysis of exosome-specific CD9, CD63, CD81, and TSG101 protein expressions in USCs and USCs-Exo