Fig. 5.

USCs-Exo promoted the proliferation and differentiation of SCs. A EdU staining after 24-h stimulation. Proliferative cells were stained green with Edu. All live cells were stained blue with hoechst. B-a Proliferation ratio of SCs in the EdU test. B-b CCK-8 test showed that USCs-Exo could promote the proliferation of SCs. B-c Myotube formation of SCs was shown as fusion index. C Twenty-four hour cultivation after USCs-Exo application. The myotube formation was promoted in the USCs-Exo group (C-a, C-b, C-c). DES was stained green and MHC was stained red. Nuclei were stained blue. Myotubes were observed as stained DES, MHC, and arrowheads in bright field (BF) (*P < 0.05, **P < 0.01; × 100 magnification)