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. 2019 Mar 8;10:80. doi: 10.1186/s13287-019-1182-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The inhibition of ERK blocked the proliferation and proliferation of SCs. A-a Phosphorylation of ERK was promoted in the USCs-Exo group. A-b Expressions of mRNAs downstream the ERK pathway were promoted in the USCs-Exo group. B After 24 h of using ERK inhibitor, PD98059, the percentage of proliferative SCs decreased obviously, as indicated by the EdU test. C-a Proliferation ratio of SCs in the EdU test. C-b CCK-8 test showed that PD98059 inhibited the proliferation of SCs. D After 24 h of using PD98059, no myotubes were observed in the PD98059 or USCs-Exo + PD98059 group compared with the USCs-Exo group (D-a, D-b, D-c). DES was stained green and MHC was stained red. Nuclei were stained blue. Myotubes were observed as stained DES, MHC, and arrowheads in bright field (BF). E Western blot analysis detected the expression of myogenic proteins in SCs under different cultivation conditions (× 100 magnification)