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. Author manuscript; available in PMC: 2019 Mar 9.

Published in final edited form as: J Am Chem Soc. 2018 Feb 20;140(8):2781–2784. doi: 10.1021/jacs.7b13660

Figure 1. — Incubation-time (t) dependence of ThT fluorescence for (a) WT Aβ40 and (b) E22G Aβ40 without seed (■) and with Aβ42 fibril seed at 5% w/w () with fitting curves to kinetic models (solid lines). The WT Aβ40 data showed little difference in the lag time (τ) between the unseeded (6.8 h) and seeded (6.2 h) samples in (a), where τ values were determined by fitting to a sigmoidal equation y(t) = a/{1+exp[−k(t-t_C)]} and τ = t_C −2/k. Unseeded E22G Aβ40 data in (b) showed intrinsically faster misfolding (τ = 1.7 h) than WT and fit with a sigmoidal curve (black line). The seeded E22G Aβ40 data in (b) showed no lag time, and fit well with the curve based on first-order kinetics equation y(t) =a[1 - exp(−kt)] (red line).

Inline graphic — Incubation-time (t) dependence of ThT fluorescence for (a) WT Aβ40 and (b) E22G Aβ40 without seed (■) and with Aβ42 fibril seed at 5% w/w () with fitting curves to kinetic models (solid lines). The WT Aβ40 data showed little difference in the lag time (τ) between the unseeded (6.8 h) and seeded (6.2 h) samples in (a), where τ values were determined by fitting to a sigmoidal equation y(t) = a/{1+exp[−k(t-t_C)]} and τ = t_C −2/k. Unseeded E22G Aβ40 data in (b) showed intrinsically faster misfolding (τ = 1.7 h) than WT and fit with a sigmoidal curve (black line). The seeded E22G Aβ40 data in (b) showed no lag time, and fit well with the curve based on first-order kinetics equation y(t) =a[1 - exp(−kt)] (red line).