Figure 7.

Low concentrations of metformin (MET) and buformin (BUF) alone or in combination with WZB-117 (WZB) do not impair mitochondrial function nor induce cell death. (a) Cells were treated with the indicated concentrations of drugs for 48 h, and the expression of TFAM and SOD2 was analyzed by Western blotting. GADPH was used as loading control. (b) Apoptosis, necroptosis, or autophagy inhibitors did not prevent the inhibitory effect of BUF+WZB on the viability in H460 cells. Cells growing under RCCs were incubated with BUF (0.5 mM)+WZB (25 μM) alone or in the presence of zVAD, Nec1, or CQ for 72 h. Cell viability was measured by the MTT assay. Results (X ± SD) are representative of two independent experiments performed in sextuplicates. (c) Low concentrations of BUF alone or in combination with WZB decreased the expression of pAMPKα. Cells were treated with the indicated concentrations of drugs for 24 or 48 h, and the expression of AMPKα and pAMPKα was analyzed by Western blotting. GADPH was used as loading control.