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. 2019 Feb 21;2019:3231784. doi: 10.1155/2019/3231784

Figure 3.

Figure 3

wLL inhibits the expression of TRAF-6 in RANKL-induced RAW 264.7 cells. RAW 264.7 cells were seeded at 2 × 106/well on a 60 mm culture plate. After 24 hours, RAW 264.7 cells were incubated with RANKL (100 ng/ml) for indicated times with or without the presence of LL (100 μg/mL). The cells were lysed with RIPA buffer, and expression of TRAF-6 was evaluated with western blot. (A) Expression of TRAF-6. (B) Expression of TRAF-6 normalized to actin. Data represent the mean ± SEM of the three independent experiments.