Figure 5.
wLL inhibits expression of osteoclast-related genes of RANKL-induced RAW 264.7 cells. RAW 264.7 cells were seeded at 5 × 105/well on a 60 mm culture plate. After 24 hours, RAW 264.7 cells were incubated with RANKL (100 ng/ml) for 4 days with or without the presence of LL (1, 10, and 100 μg/mL). The cells were extracted using a TriZol kit, and mRNA expression of osteoclast-related genes was determined by RT-PCR. (a) mRNA expressions of osteoclast-related genes. (b) mRNA expressions normalized to GAPDH. Data represent the mean ± SEM of the three independent experiments.