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. 2019 Feb 21;2019:3231784. doi: 10.1155/2019/3231784

Table 1.

Primers, sequences, and PCR conditions.

Primer name Primer Sequence Annealing Tm (°C) cycle
TRAP 5′-ACTTCCCCAGCCCTTACTACCG-3′ 
 5′-TCAGCACATAGCCCACACCG-3′
58°C 32
NFATc1 5′-TGCTCCTCCTCCTGCTGCTC-3′ 
 5′-CGTCTTCCACCTCCACGTCG-3′
58°C 32
c-Fos 5′-ATG GGC TCT CCT GTC AAC AC-3′ 
 5′-GGCTGCCAA AATAAACTCCA-3′
58°C 33
RANK 5′-AAACCTTGGACCAACTGCAC-3′ 
 5′-ACCATCTTCTCCTCCCGAGT-3′
53°C 32
CAII 5′-CTCTCAGGACAATGCAGTGCTGA-3′ 
 5′-ATCCAGGTCACACATTCCAGCA-‘3
58°C 33
CTK 5′-AGGCGGCTATATGACCACTG-3′ 
 5′-CCGAGCCAAGAGAGCATATC-‘3
58°C 28
OSCAR 5′-CTGCTGGTAACGGATCAGCTCC CCAGA-3′ 
 5′-CCAAGGAGCCAGAACCTTCGAAACT-3′
53°C 40
ATP6v0d2 5′-ATGGGGCCTTGCAAAAGAAATCTG-3′ 
 5′-CGACAGCGTCAAACAAAGGCTTGTA-3
58°C 30
GAPDH 5′-ACTTTGTCAAGCTCATTTCC-3′ 
 5′-TGCAGCGAACTTTATTGATG-3′
57°C 30