Table 3.
The liquid chromatography gradient elution method.
| Time (min) | A (%) | B (%) |
|---|---|---|
| 0 | 95 | 5 |
| 1.5 | 95 | 5 |
| 3 | 80 | 20 |
| 5 | 40 | 60 |
| 7 | 5 | 95 |
| 10 | 5 | 95 |
| 10.1 | 95 | 5 |
| 12 | 95 | 5 |
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