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Effect of Rg3 on EMT-related transcription factors expression in HNE1 and CNE2 cells. (a) mRNA levels of Snail, Slug, Twist, and ZEB1 were analysed by RT-PCR. (b, d) Protein levels of Snail, Slug, Twist, and ZEB1 were analysed by Western blot. (c, e) Relative protein expression of Snail, Slug, Twist, and ZEB1 in HNE1 and CNE2 cells. Results are expressed as mean ± SD (n=3). ∗∗P<0.01 vs Control and ∗∗∗P<0.001 vs Control.
