Figure 1.

Characterization of hMSCs was confirmed. (a) Density gradient separation of human bone marrow: (A) plasma, (B) mononuclear cells, (C) ficoll paque, (D) erythrocytes, and granulocytes. (b) Morphology of replated cells showed homogeneous and fibroblastic shape. (c) Representative images of multicolour CD markers by flow cytometry. The results showed that hMSCs expressed at least 90% of double-positive expression, double-negative, or coexpressed positive and negative markers. (d) Trilineage differentiation potential of the hMSCs into osteogenic, adipogenic, and chondrogenic lineage.