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. 2019 Feb 21;2019:9723025. doi: 10.1155/2019/9723025

Figure 5.

Figure 5

Mechanical stretching altered hMSC surface antigen expression but did not express osteogenesis and adipogenesis. (a) Expression levels of the CD markers of hMSCs cultured in mechanical stretching with different strains. Significance (p < 0.05) was represented by which compared to unstrained. n = 3, error bar ± SD. (b) Percentage of multicolour expression for lymphocyte adhesion molecule CD44 and endoglin CD105. Fluorescent expression intensity and area of CD44 and CD105 in (A) 4%, (B) 8%, and (C) 12% strain magnitudes. (c) Representative images of Alizarin Red- and Oil Red O-stained hMSCs: (A) positive Alizarin Red staining on osteogenic medium cultured hMSCs, (B) negative Alizarin Red result on mechanical stimulated hMSCs, (C) positive Oil Red O staining on adipogenic medium cultured hMSCs, (D) negative Oil Red O result on strained hMSCs.