(A) IB analysis of whole cell lysates from untreated cells. (B) Effects of PEPDG278D and Ttzm on cell proliferation, measured by MTT assay. Each value is mean ± SD (n=3). ****P<0.0001 by one-way ANOVA, followed by Tukey test for comparison to the control. (C) IB analysis of whole cell lysates from cells treated with vehicle, PEPDG278D (25 nM) or Ttzm (1 μM) for 48 hours. The following phosphorylation sites were measured: pY1221/1222-HER2, pY1173-EGFR, pY416-SRC, pS473-AKT, pT202/Y204-ERK, pY1131-IGF1R, and pY1234/1235-MET. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was measured as a loading control here and elsewhere.