Table 1.
Experiment | Nerve Label | Surgical Conditions | Time of Nerve Label | Tissue Extraction | Variable Assessed |
---|---|---|---|---|---|
CT Terminal Field | BDA | P5 CTX (n = 5) P10 CTX (n = 5) Sham (n = 5) |
50-51 Post Surgery | 48 hr After Label | Terminal Field Volume Dorsal-Ventral Extent of Field |
DiI | P5 CTX (n = 6) P50 CTX (n = 6) |
Immediately After CTX | 50-51 Days Post-Surgery | Terminal Field Volume | |
CT Ganglion Cell Counts | DiI | P5 CTX (n = 5)a P50 CTX (n = 4)a |
Immediately After CTX | 50-51 Days Post-Surgery | Number of Labeled GG Neurons |
GL-GSP Nerve Label | GL – TMR GSP – BDA |
P5 CTX (n = 5) P10 CTX (n = 5) No surgery (n = 5) |
50 Days After Surgery | 48 hr After Label | Terminal Volume Terminal Field Overlap |
GG Neuron Counts (aged rats) | N/A | P10 CTX (n = 5) | N/A | 567-937 Days Post-Surgery | GG Neurons Ipsilateral to CTX vs. GG Neurons Contralateral to CTX |
These animals are a subset of those used to assess CT terminal field using DiI.