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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Neuroscience. 2019 Jan 24;402:66–77. doi: 10.1016/j.neuroscience.2019.01.011

Table 1.

Experimental Design

Experiment Nerve Label Surgical Conditions Time of Nerve Label Tissue Extraction Variable Assessed
CT Terminal Field BDA P5 CTX (n = 5)
P10 CTX (n = 5)
Sham (n = 5)
50-51 Post Surgery 48 hr After Label Terminal Field Volume
Dorsal-Ventral Extent of Field
DiI P5 CTX (n = 6)
P50 CTX (n = 6)
Immediately After CTX 50-51 Days Post-Surgery Terminal Field Volume
CT Ganglion Cell Counts DiI P5 CTX (n = 5)a
P50 CTX (n = 4)a
Immediately After CTX 50-51 Days Post-Surgery Number of Labeled GG Neurons
GL-GSP Nerve Label GL – TMR
GSP – BDA
P5 CTX (n = 5)
P10 CTX (n = 5)
No surgery (n = 5)
50 Days After Surgery 48 hr After Label Terminal Volume
Terminal Field Overlap
GG Neuron Counts (aged rats) N/A P10 CTX (n = 5) N/A 567-937 Days Post-Surgery GG Neurons Ipsilateral to CTX vs. GG Neurons Contralateral to CTX
a

These animals are a subset of those used to assess CT terminal field using DiI.