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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Gastroenterology. 2018 Dec 3;156(4):1052–1065. doi: 10.1053/j.gastro.2018.11.061

Figure 3. Effects of fasting and refeeding on intestinal expression of NPC1L1 in WT and FGF15-knockout mice.

Figure 3.

(A-C) WT and FGF15-knockout (KO) mice were fasted overnight and refed normal chow for 6 h and mRNA (A) and protein (B) levels in the pooled jejunum and ileum were measured. (C) NPC1L1 protein was detected by immunohistochemistry in sections of the jejunum. (D) WT and FGF15-knockout mice were fasted overnight and refed normal chow for 3 or 6 h, and pre-mRNA levels in the pooled jejunum and ileum were determined by RT-qPCR. (E-F) WT and SHP-knockout mice were fasted overnight and injected with FGF19 (1 mg/kg) via the tail vein and sacrificed 6 h later. Levels of Npc1l1 mRNA (E) and protein (F, left and middle) in the pooled jejunum and ileum were determined by RT-qPCR and IB, respectively. (F, right) NPC1L1 protein was detected by IHC in sections of the jejunum. (A, B, D, E, F) Statistical significance was determined by (A, B, E, F) two-way or (D) one-way ANOVA with the FDR post-test, (SEM, n=3 to 5, *P <0.05, **P <0.01, NS, statistically not significant).