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. 2019 Mar 4;6:51. doi: 10.3389/fvets.2019.00051

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Copyright © 2019 Küchler, Koch, Seuberlich and Boujon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry (IHC) for BoAstV-CH15/ OvAstV-CH16 in sheep 21979 and corresponding histopathological lesions. IHC using hyperimmune antiserum CH15-ORF2-var (A,C) and hematoxylin-eosin staining of corresponding areas (B,D). (A) Cerebral cortex; the inset on the upper left shows the marked area at a higher magnification. (B) Cerebral cortex; apparent perivascular cuff on the lower left. (C) Cerebellum; arrowheads point out positively-stained Purkinje cells. (D) Cerebellum; pronounced perivascular cuff on the right. (E) IHC using hyperimmune antiserum CH15-ORF2-con, showing positive staining in the diencephalon of sheep 21979.