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. 2019 Mar 4;13:41. doi: 10.3389/fnbeh.2019.00041

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Copyright © 2019 Lundberg, Högman and Roman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental outline and behavioral test arenas with defined zones. (A) Experimental outline with rat developmental stages and age in weeks (NB, newborn) (Tirelli et al., 2003; Alberts, 2005; Spear, 2015). Schematic layout of the (B) multivariate concentric square field^TM (MCSF), (C) elevated plus maze (EPM), and (D) open field (OF) (with start box) arenas. The arenas are divided into zones by walls (solid, black lines) or imagined boundaries (dashed lines). MCSF zones: (1) center, (2) central circle (CTRCI), (3a–c) corridors, (4) dark corner room (DCR), (5) hurdle, (6) slope, (7) bridge entrance, and (8) bridge. EPM zones: (a) open arms, (b) closed arms, and (c) central square (CTRSQ). OF zones: (I) outer circle (OC), (II) inner circle (IC), (III) center (CTROF) and, when used, (IV) start box. The saturation level in the figures reflects the approximate illumination levels in the arenas.