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. 2019 Mar 4;10:257. doi: 10.3389/fimmu.2019.00257

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Copyright © 2019 Rudolph, McArthur, Magder, Barnes, Chen and Sztein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD8+ T Effector Memory RA Responses. Scatter plots showing (A) the responses over baseline percentages of CD8+ CD69+ T_EMRA producing/expressing MIP1β, CD107a, TNFα, IL-2, IL-17A, IFNγ, and Granzyme B (GzmB); (B) CD8+ CD69+ T_EMRA observed mono-, bi-, tri-, and >3-functional populations; and (C). specific CD8+ CD69+ T_EMRA selected multifunctional responses following co-culture with HLA-E restricted S. Typhi infected antigen presenting targets. These MF populations were selected based on those shown in a previous S. Typhi challenge study to be associated with protection from developing disease (10, 11). Bars represent medians with whiskers indicating interquartile ranges. Results are shown for the following populations: 6–15 year-old pediatric (n = 10), 16–17 year-old pediatric (n = 8), and adult (n = 13) participants. Scatter plot statistics were analyzed by unpaired t-test. (*p < 0.05).