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. 2019 Mar 4;10:257. doi: 10.3389/fimmu.2019.00257

Figure 6.

Figure 6

tSNE analysis of activated CD8+ T effector memory RA (TEMRA) populations. (A) tSNE maps of pre- and post-Ty21a vaccinated 6–15 year old, 16–17 year old, and adult participants. (B,C) Significantly different clusters between age groups (6–15 year old and 16 years and over) represented by bar graphs identifying the average percent of cells per volunteer per cluster in pre-Ty21a (B) and post-Ty21a (C) immunization timepoints. (D–G) Functions of significantly different tSNE clusters from (B,C), divided into: (D) median expression of Tel effector functions MIP1β, CD107a, TNFα, IL-2, IFNγ, and Granzyme B (GzmB), (E) Winlist FCOM-analysis determined Tc1 mono- and multifunctional cytokine expression, (F) median expression of homing markers, and (G) median expression of non-Tc1 associated cytokines. (H) Clusters showing significant net changes in the percent of cells following Ty21a vaccination. (I–L) Functions of significantly different tSNE clusters from (H), divided into: (I) median expression of Tc1 effector functions MIP1β, CD107a, TNFα, IL-2, IFNγ, and Granzyme B (GzmB), (J) Winlist FCOM-analysis determined Tc1 mono- and multifunctional cytokine expression, (K) median expression of homing markers, and (L) median expression of non-Tc1 associated cytokines.