Table 2.
Mass cytometry panel showing antibody target, stable metal isotope (or other label), antibody clone, and a brief description of the target function.
| Target | Stable metal isotope | Clone | Description |
|---|---|---|---|
| CD14 | 114 Cd (Qdot) | TüK4 | Monocyte marker |
| CD19 | 114 Cd (Qdot) | SJ25-C1 | B cell marker |
| IL-4 | 142 Nd | MP425D2 | Tc/h2 effector cytokine |
| CXCR5 | Biotin | RF8B2 | Follicular homing chemokine receptor |
| Biotin | 143 Nd | 1D4-C5 | |
| α4β7 | FITC | ACT-1 | Gut-homing integrin |
| FITC | 144 Nd | FIT22 | |
| CD8 | 146 Nd | RPA-T8 | Cytotoxic T lymphocyte marker |
| IL-6 | 147 Sm | MQ213A5 | Proinflammatory proliferation-associated cytokine |
| CCR4 | 149 Sm | L291H4 | Chemokine homing to the skin |
| MIP-1β | 150 Nd | D21-1351 | NK and monocyte recruiting chemokine |
| CD107a | 151 Eu | H4A3 | Degranulation marker |
| TNFα | 152 Sm | Mab11 | Proinflammatory cytokine |
| CD62L | 153 Eu | DREG-56 | Lymphoid-tissue homing selectin |
| CD45 | 154 Sm | HI30 | Pan-leukocyte barcoding marker |
| CD27 | 155 Gd | L128 | TNF superfamily—costimulatory molecule |
| CD45 | 156 Gd | HI30 | Pan-leukocyte barcoding marker |
| IL-2 | 158 Gd | MQ1-17H12 | Induction of T cell differentiation and proliferation |
| CD69 | 162 Dy | FN50 | Activation marker |
| CXCR3 | 163 Dy | G025H7 | Chemokine homing to sites of inflammation |
| IL-17A | 164 Dy | N49-653 | Tc/h17 effector cytokine |
| IFNγ | 165 Ho | B27 | Proinflammatory cytokine |
| IL-10 | 166 Er | JES3-9D7 | Anti-inflammatory cytokine |
| CD154 (CD40L) | 168 Er | 24-31 | Co-stimulatory molecule; Tfh induction of B cell maturation |
| CD45RA | 169 Tm | HI-100 | T cell memory marker |
| CD3 | 170 Er | UCHT1 | TCR co-receptor (T cell marker) |
| Granzyme B | 171 Yb | GB11 | Secreted cytotoxic effector molecule |
| IL-21 | 172 Yb | 3A3-N2 | Tfh effector cytokine (germinal center formation) |
| ICOS (CD278) | 173 Yb | C398.4A | Tfh co-stimulatory marker (B cell help) |
| CD4 | 174 Yb | SK3 | Helper T lymphocyte marker |
| PD-1* | 175 Lu | EH12.2H7 | T cell exhaustion/activation marker |
| CCR6 | 176 Yb | G034E3 | Chemokine homing to mucosal surfaces |
| Cell I.D. (DNA) | 191/193 Ir | n/a | DNA intercalator |
| Viability | 194/195 Pt | n/a | Viability stain |
Primary surface antibodies are filled with blue, secondary surface antibodies with green, and intracellular antibodies with orange (*anti-PD-1 is in both the surface and intracellular antibody mixes). Barcoding antibodies are shaded in yellow, and anti-CD107a, which is added during stimulation to best account for the active cycling between the surface and intracellular vesicles, is highlighted in purple.