Figure 2.

Upregulation of miR-582-3p and miR-582-5p Represses Bone Metastasis of PC-3 Cells In Vivo

(A) Representative BLIs signal of bone metastasis of a mouse from the vector or pri-miR-582 groups of mice at 5 min and 45 days, respectively. (B) Representative radiographic images of bone metastases in the indicated mice (blue arrows indicate osteolytic lesions). (C) Representative H&E-stained sections of tibias from the indicated mouse. (D) The sum of bone metastasis scores for each mouse in tumor-bearing mice inoculated with vector (n = 9) or pri-miR-582 (n = 11) cells. (E) Quantification of the ROI signaling in the vector and pri-miR-582 groups at 6, 7, and 8 weeks, respectively. (F and G) Kaplan-Meier analysis of mouse survival (F) and bone metastasis-free survival (G) in the vector and pri-miR-582 groups. (H) Real-time PCR analysis of miR-582-3p and miR-582-5p expression in the tumor tissues of the mice inoculated with PC-3 cells stably transfected with pri-miR-582 compared with those in the vector at the end of the experiments. Transcript levels were normalized by U6 expression. Error bars represent the mean ± SD of three independent experiments. *p < 0.05.