Upregulation of miR-582-3p or miR-582-5p Represses Invasion and Migration Abilities of PCa Cells In Vitro
(A) Real-time PCR analysis of miR-582-3p or miR-582-5p expression in PCa cells transduced with miR-582-3p or miR-582-5p mimics, respectively, compared with the vector controls. Transcript levels were normalized by U6 expression. Error bars represent the mean ± SD of three independent experiments. (B) Overexpression of miR-582-3p inhibited invasion and migration abilities in PCa cells. Error bars represent the mean ± SD of three independent experiments. (C) Overexpression of miR-582-5p inhibited invasion and migration abilities in PCa cells. Error bars represent the mean ± SD of three independent experiments. (D) Western blotting of ZEB2, SNAI2, and MMP13 expression in the indicated PCa cells. α-Tubulin served as the loading control. *p < 0.05.